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Results: 12

au:"Silveira, José Franco da"

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Mariano Jorge Levin (1951-2010)
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Silveira, José Franco da

Revista do Instituto de Medicina Tropical de São Paulo

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apr 2010, Volume 52 N. 2 Pages 115 - 115

Abstract: En | Text: En | PDF: En

1122 downloads

Analysis and chromosomal mapping of Leishmania (Leishmania) amazonensis amastigote expressed sequence tags
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Gentil, Luciana Girotto

; Lasakosvitsch, Fernanda

; Silveira, José Franco da

; Santos, Márcia Regina Machado dos

; Barbiéri, Clara Lúcia

Memórias do Instituto Oswaldo Cruz

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sep 2007, Volume 102 N. 6 Pages 707 - 711

Abstract: En | Text: En | PDF: En

The characterization of expressed sequence tags (ESTs) generated from a cDNA library of Leishmania (Leishmania) amazonensis amastigotes is described. The sequencing of 93 clones generated new L. (L.) amazonensis ESTs from which 32% are not related to any other sequences in database and 68% presented significant similarities to known genes. The chromosome localization of some L. (L.) amazonensis ESTs was also determined in L. (L.) amazonensis and L. (L.) major. The characterization of these ESTs is suitable for the genome physical mapping, as well as for the identification of genes encoding cysteine proteinases implicated with protective immune responses in leishmaniasis.

1732 downloads

A novel reiterated family of transcribed oligo(A)-terminated, interspersed DNA elements in the genome of Trypanosoma cruzi
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Verbisck, Newton Valério

; Santos, Márcia Regina Machado dos

; Engman, David M

; Angel Chiurillo, Miguel

; Ramirez, José Luis

; Araya, Jorge Enrique

; Mortara, Renato Arruda

; Silveira, José Franco da

Memórias do Instituto Oswaldo Cruz

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jan 2003, Volume 98 N. 1 Pages 129 - 133

Abstract: En | Text: En | PDF: En

We report the molecular characterization of a novel reiterated family of transcribed oligo(A)-terminated, interspersed DNA elements in the genome of Trypanosoma cruzi. Steady-state level of transcripts of this sequence family appeared to be developmentally regulated, since only in the replicative forms the parasite showed expression of related sequences with a major band around 3 kb. The presence of frame shifts or premature stop codons predicts that transcripts are not translated. The sequence family also contains truncated forms of retrotransposons elements that may become potential hot spots for retroelement insertion. Sequences homologous to this family are interspersed at many chromosomes including the subtelomeric regions.

2634 downloads Cited 1 time in SciELO

Structure and transcription of genes encoding the surface glycoprotein antigens gp90 and gp82 of Trypanosoma cruzi metacyclic trypomastigotes
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Carmo, Mirian S.

; Araya, Jorge E

; Cano, Maria I.

; Ramirez, Marcel I.

; Baida, Renata P.

; Ruiz, Rita C.

; Santos, Marcia R.

; Chiurillo, Miguel A.

; Ramirez, José L.

; Yoshida, Nobuko

; Silveira, José Franco da

Anais da Academia Brasileira de Ciências

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mar 2000, Volume 72 N. 1 Pages 104 - 105

Abstract: En | Text: En | PDF: En

1160 downloads

Organization and expression of a multigene family encoding the surface glycoproteins of Trypanosoma cruzi metacyclic trypomastigotes involved in the cell invasion
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Carmo, Mirian S

; Araya, Jorge E

; Ramirez, Marcel I

; Boscardin, Silvia

; Cano, Maria I

; Baida, Renata P

; Ruiz, Rita C

; Santos, Marcia R

; Chiurillo, Miguel A

; Ramirez, José L

; Yoshida, Nobuko

; Silveira, José Franco da

Memórias do Instituto Oswaldo Cruz

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sep 1999, Volume 94 Suppl. 1 Pages 169 - 171

Abstract: En | Text: En | PDF: En

1794 downloads

Serological diagnosis of Chagas disease with purified and defined Trypanosoma cruzi antigens
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Umezawa, Eufrosina Setsu

; Silveira, José Franco da

Memórias do Instituto Oswaldo Cruz

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sep 1999, Volume 94 Suppl. 1 Pages 285 - 288

Abstract: En | Text: En | PDF: En

2361 downloads Cited 5 times in SciELO

Heterologous Expression of a Trypanosoma cruzi Surface Glycoprotein (gp82) Indicates that Requirements for Glycosylphosphatidylinositol Anchoring are Different in Mammalian Cells and this Trypanosome
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Ramirez, Marcel I

; Boscardin, Silvia B

; Ruiz, Rita C

; Han, Sang W

; Paranhos-Baccala, Glaucia S

; Yoshida, Nobuko

; Mortara, Renato A

; Silveira, José Franco da

Memórias do Instituto Oswaldo Cruz

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jul 1999, Volume 94 N. 4 Pages 527 - 530

Abstract: En | Text: En | PDF: En

1731 downloads

The Trypanosoma cruzi Genome Project: Nuclear Karyotype and Gene Mapping of Clone CL Brener
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Santos, Marcia RM

; Cano, Maria I

; Schijman, Alejandro

; Lorenzi, Hernan

; Vázquez, Martin

; Levin, Mariano J

; Ramirez, José Luis

; Brandão, Adeílton

; Degrave, Wim M

; Silveira, José Franco da

Memórias do Instituto Oswaldo Cruz

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nov 1997, Volume 92 N. 6 Pages 821 - 828

Abstract: En | Text: En | PDF: En

By using improved pulsed field gel electrophoresis conditions, the molecular karyotype of the reference clone CL Brener selected for Trypanosoma cruzi genome project was established. A total of 20 uniform chromosomal bands ranging in size from 0.45 to 3.5 Megabase pairs (Mbp) were resolved in a single run. The weighted sum of the chromosomal bands was approximately 87 Mbp. Chromoblots were hybridized with 39 different homologous probes, 13 of which identified single chromosomes. Several markers showed linkage and four different linkage groups were identified, each comprising two markers. Densitometric analysis suggests that most of the chromosomal bands contain two or more chromosomes representing either homologous chromosomes and/or heterologous chromosomes with similar sizes

3386 downloads Cited 1 time in SciELO

Parasite Genome Projects and the Trypanosoma cruzi Genome Initiative
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Degrave, Wim

; Levin, Mariano J

; Silveira, José Franco da

; Morel, Carlos M

Memórias do Instituto Oswaldo Cruz

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nov 1997, Volume 92 N. 6 Pages 859 - 862

Abstract: En | Text: En | PDF: En

Since the start of the human genome project, a great number of genome projects on other "model" organism have been initiated, some of them already completed. Several initiatives have also been started on parasite genomes, mainly through support from WHO/TDR, involving North-South and South-South collaborations, and great hopes are vested in that these initiatives will lead to new tools for disease control and prevention, as well as to the establishment of genomic research technology in developing countries. The Trypanosoma cruzi genome project, using the clone CL-Brener as starting point, has made considerable progress through the concerted action of more than 20 laboratories, most of them in the South. A brief overview of the current state of the project is given

1593 downloads Cited 2 times in SciELO

10.

Antigens of Trypanosoma cruzi with clinical interest cloned and expressed in Escherichia coli
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Silveira, José Franco da

; Paranhos, Gláucia S.

; Cotrim, Paulo C.

; Mortara, Renato A.

; Camargo, Mário E.

; Rassi, Anis

; Wanderley, Jamiro

; Corral, Ricardo

; Freilij, Hector L.

; Grinstein, Saul

; Degrave, Win

Memórias do Instituto Oswaldo Cruz

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dec 1990, Volume 85 N. 4 Pages 507 - 511

Abstract: En | Text: En | PDF: En

1199 downloads

11.

Characterization of a Trypanosoma cruzi genomic fragment complementary to several species-specific mRNAs but different from the spliced leader sequence
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Cotrim, Paulo Cesar

; Cummings, Leda Maria

; Silveira, José Franco da

Memórias do Instituto Oswaldo Cruz

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jun 1989, Volume 84 N. 2 Pages 143 - 149

Abstract: Pt En | Text: Pt En | PDF: Pt | PDF: En

Foi isolado um clone recombinante (lambda 3b2-5) de ADN genômico de Trypanosoma cruzi, o qual contém seqüências que estão reiteradas no genoma. Análise através da técnica de "Northern blot" mostrou que o clone 3b2-5 hibridiza com diferentes espécies de ARNs mensageiros de 1.200 a 5.000 bases. O número de espécies de ARNs mensageiros que hibridiza com o clone 3b2-5 excede sua capacidade codante, sugerindo que este clone apresenta seqüências que são comuns a muitas espécies de ARNs mensageiros e conservadas no ARN poliadenilado. Estas seqüências não são homológas a seqüência líder presente nos ARNs de T. cruzi, pois, o clone 3b2-5 não hibridiza com um nucleotídeo sintético complementar a seqüência líder. O clone 3b2-5 não hibridiza com o ADN e ARN de diferentes gêneros de tripanosomatídeos e outras espécies de tripanosomas indicando que ele apresenta seqüências específicas para o T. cruzi.

We have isolated a clone of Trypanosoma cruzi genimic DNA, lambda 3b2-5, which contains sequences that are reiterated in the genome. Northtern blot analysis showed that clone 3b2-5 hybridizes to 1,200-5,000 bases different mRNA species. The number of mRNAs species hybridized to clone 3b2-5 exceeds its coding capacity showing that this clone carries sequences that are common to several mRNAs species and conserved in the poly A(+) RNA. These sequences are not homologous to the T. cruzi spliced leader sequence, since clone 3b2-5 hybridize to a synthetic 20 nucleotice complementary to the spliced leader sequence. Clone 3b2-5 does not hybridize to DNA and RNA from several genera of Trypanosomatidae and other Trypanosoma species indicating that it carries T. cruzi species-specific sequences.

1737 downloads Cited 1 time in SciELO

12.

Trypanosoma cruzi: cloning of the 26 S Ribosomal gene and its associated spacer
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Mello, Maricilda P. de

; Gomes, Henrique B. M.

; Guevara, Palmira

; Ramirez, José L.

; Silveira, José Franco da

Memórias do Instituto Oswaldo Cruz

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nov 1988, Volume 83 Suppl. 1 Pages 334 - 336

Abstract: En | Text: En | PDF: En

976 downloads

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